3/28 Loading and Running the Gel

Today we used the PCR products we ran yesterday to see if we successfully amplified the RBCL Gene. We prepared samples 20 uL of each of the PCR products mixed with 4 uL of 6x Loading Dye. We loaded each of these samples, as well as 10 uL of 10 Log Ladder into a .7% Agarose Gel.

gel

Gel Electrophoresis of Samples

We will run this gel on high for 1 hour. Then, we stained it with Sybr Gold and analyzed the gel under a UV light.

As you can see in the gel, only the ladder showed up. Therefore, we are going to redo the PCR reactions and rerun the gel.

annotated gel trial 1

Gel Results

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