4/25 Analyzing GMO Gradient Reactions/Making Master Mix

First, we reviewed the gel results of the GMO primer gradient reactions, which was pretty successful. Even though the negative control was contaminated, there was good amplification in the higher temperature reactions. To fix the issue with the contaminated negative control, we will use new water in our future reactions. We've concluded that Cereal D … Continue reading 4/25 Analyzing GMO Gradient Reactions/Making Master Mix

Advertisements

4/21 Gradient Reactions for GMO Primers

Because we had a lot of success with the Rubsico PCR gradient, we've decided to complete a PCR gradient with Dr. Todd's GMO primers. We made the following master mix for 10x reactions: 1x (uL) 10x (uL) H2O 19 190 10x 2.5 25 DNTP .5 5 Forward Primer 1 10 Reverse Primer 1 10 Taq … Continue reading 4/21 Gradient Reactions for GMO Primers