Today we loaded the gel. First, we added 5μL of 6x dye to each of the 6 PCR tubes. Then, we loaded 10μL of 2 log ladder into the first lane. We skipped the second well, and then loaded the 6 PCR reactions in order in lanes 3-8.
We ran the gel on high for about 45 minutes and then stained the gel using Sybr Gold stain. Finally, we observed the gel under a UV light, and this time, there were conclusive results!