4/4 Extracting more DNA from Oats and PCR Trial

Today, we extracted more DNA from the oats, but we used a slightly different protocol – “Try 2 – Preparing the Negative Control”. This new protocol allowed for us to get actual DNA, instead of an “oat-fiber” mixture.

We also redid “Trial 2 – PCR Reactions” and “Trial 2 – Running the Gel”.

Michael spent the class looking up a new primer sequence for the positive control.

IMG_8973

Michael and Dr. Todd Looking for Primers

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s