Today, Lindsay came before class and loaded our 0.7% agarose gel. 4 μL of 6x loading dye was added to 20μL of each PCR reaction. In lane 1, 10μL of 10 log ladder was loaded. Lane 2 was left empty. In lanes 3-7, PCR reactions 1-5 were loaded. Lane 8 was left empty. In lanes 9-13, PCR reactions 6-10 were loaded. The gel was run on high for 40 minutes.
After the gel had run, we stained the gel in Cybr gold on a rocker.