4/19 Running/Analyzing the Gel

Today, Lindsay came before class and loaded our 0.7% agarose gel.  4 μL of 6x loading dye was added to 20μL of each PCR reaction. In lane 1, 10μL of 10 log ladder was loaded. Lane 2 was left empty. In lanes 3-7, PCR reactions 1-5 were loaded. Lane 8 was left empty. In lanes 9-13, PCR reactions 6-10 were loaded. The gel was run on high for 40 minutes. 

happy lindsay

Happy Lindsay

gel running

Gel Running

After the gel had run, we stained the gel in Cybr gold on a rocker.

rocker

Staining the Gel

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s