Today we planned our next steps of the experiment. We are going to preform a gradient PCR reactions to determine the true optimal temperature to get the Rubisco primers to cut our food samples. We decided that will will do a gradient PCR reaction with Cereal D because Cereal D was cut the best by the Rubisco Primers (see gel image below).
We made the following master mix for 10x reactions:
|1x (uL)||10x (uL)|
|*take out one reaction for neg control*|