5/1 Making the PAGE Gel

Today, we worked with Dr. Todd on the procedure for making a PAGE gel (Polyacrylamide gel electrophoresis) to run our PCR samples in.  This was a new skill for our group members and that was somewhat evident.  We hit some road blocks due to our inexperience.  We used Accogel, 50x TAE and sterile water to make the solution to start.  Lastly, 100 ul of a 10% ASP solution and 5 ul of TEMED.  At the end of the class, the final solution was poured to harden.  We hope to load our samples into it tomorrow.

making-page-gel.jpg

Dr. Todd Pouring PAGE Gel

 

 

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