5/4 New DNA Extraction Protocol

Today Danielle and Jess followed  a new DNA extraction protocol in hopes to better the quality of the DNA we get for the PCR reactions. We followed the protocol below:

  1. Put in ¼ flake
  2. Vortex and grind w pestle
  3. Add 300μL 1% saline solution
  4. Vortex
  5. Microfuge 2 min
  6. Take all supernatant and put in 300μL chelex
  7. Vortex again 2 min
  8. 10 min at 95 degrees in hot water bath
  9. Spin for 2 min
  10. Take 50μL supernatant into new tube
new dna extraction protocol

Protocol

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