5/9 Loading/Running the Final Gel

With the final PCR done, the next step was to pour a gel and load the samples onto it to see if the PCR worked. Dr. Todd already poured the gel when we came in, so we poured TAE buffer into the gel running box and the gel. We removed the well combs and loaded the samples. The marker solution had 10ul of marker and 2ul of 6x dye and was loaded on the first lane of both rows. The second lanes were skipped and lanes 3-8 had 20ul of the sample and 4ul of 6x dye. Lanes 6-8 were loaded poorly due to an issue with the pipettes. After loading, the gel was run on high for 40 minutes and then stained and viewed under a UV light.

 

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